Polymerase Chain Reaction Study of Human Bocavirus in Children with Acute Gastroenteritis

Aim: The present study aimed to detect Human bocavirus (HBoV) in stool samples from young children below 5 years complaining of acute gastroenteritis (GE) addition detection rotavirus, norovirus, and astrovirus. Methods: included 90 with GE excluded bacterial pathogens. determination the presence HBoV was performed by nested polymerase chain reaction (PCR) beside astrovirus norovirus multiplex PCR rotavirus antigen enzyme-linked immunosorbent assay (ELISA). Results: most prevalent virus among studied viruses (33.3%) detected ELISA for stool. other three molecular methods were (14.4%), (13.3%), (10%). Mixed viral infection two or more 16 (17.8%). common 6 patients (37.5%). In demographic clinical presentations HBoV, affected mainly females, i.e ., 10 (76.9%), rural residence 11 (84.6%) mild classification 7 (53.8%) moderate (46.2%) none them had severe GE. Fever presenting sign those (53.8% ) followed vomiting (46.2%). Conclusion: highlights existence under age five. associated this either severity. combined especially rotavirus. There is a need further additional identify type circulated strain confections